Shared Equipment in 315 Worrilow

Shared Equipment in 315 WORRILOW

BioRAD ChemiDoc MP imaging system

The ChemiDoc MP Imaging System is a full-feature instrument for imaging and analyzing gels and western blots. It addresses multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications and stain-free technology imaging needs. The accompanying Image Lab Software (free stand-alone versions available for download from BioRad) allows for band identification, volume analysis and normalization of band density.

Thermo MaxQ 800

This platform shaker allows for the growth of cultures in different formats (tubes up to 1 liter flasks) in a temperature controlled environment at a variety of speeds. Ideal for growing bacterial cultures, the rapid temperature controller is ideal for heat-inducible bacterial expression systems.

SpectraMax iD5

The Molecular Devices Spectramax ID5 Plate Reader from Molecular Devices can measure absorbance (230 - 1000 nm), fluorescence (excitation: 250 - 830 nm, emission: 270 - 850 nm) and fluorescence polarization, luminescence (300 - 650 nm), and time-resolved phosphorescence. The plate reader can accommodate both 96 and 384-well plate formats. It also has dual substrate injectors for kinetic assays using firefly and renilla luciferases.

Axygen Gel Documentation System

The Axygen Gel Documentation system allows for the capture of 16-bit TIFF images and features an easy-to-use interface for image capture, annotation and contrast adjustment. Images can be saved for analysis in standard gel software.

Molecular Devices SpectraMax M2

The Molecular Devices Spectramax M2 plate reader is a multi-detection microplate reader with dual-monochromators, dual-mode cuvette ports and top-reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The instrument does endpoint, kinetic, spectrum and area-well scanning read types and is used for DNA/RNA/protein quantification, Bradford, PicoGreen and NanoOrange, ELISA and enzyme kinetics assays.

Thermo Nanodrop One

This instrument is essential for accurately measuring DNA, RNA and protein for direct applications such as real-time qRT-PCR, western blots, high-throughput sequencing, etc. Another benefit is its small footprint, which can easily fit on the benchtop among other equipment. It is also easily cleanable and care is simple, as long as people take a little time.