Shared Equipment in 315 Worrilow
Shared Equipment in 315 WORRILOW
BioRAD ChemiDoc MP imaging system
The ChemiDoc MP Imaging System is a full-feature instrument for imaging and analyzing gels and western blots. It addresses multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications and stain-free technology imaging needs. The accompanying Image Lab Software (free stand-alone versions available for download from BioRad) allows for band identification, volume analysis and normalization of band density.
Thermo MaxQ 800
This platform shaker allows for the growth of cultures in different formats (tubes up to 1 liter flasks) in a temperature controlled environment at a variety of speeds. Ideal for growing bacterial cultures, the rapid temperature controller is ideal for heat-inducible bacterial expression systems.
SpectraMax iD5
The Molecular Devices Spectramax ID5 Plate Reader from Molecular Devices can measure absorbance (230 - 1000 nm), fluorescence (excitation: 250 - 830 nm, emission: 270 - 850 nm) and fluorescence polarization, luminescence (300 - 650 nm), and time-resolved phosphorescence. The plate reader can accommodate both 96 and 384-well plate formats. It also has dual substrate injectors for kinetic assays using firefly and renilla luciferases.
Axygen Gel Documentation System
The Axygen Gel Documentation system allows for the capture of 16-bit TIFF images and features an easy-to-use interface for image capture, annotation and contrast adjustment. Images can be saved for analysis in standard gel software.
Molecular Devices SpectraMax M2
The Molecular Devices Spectramax M2 plate reader is a multi-detection microplate reader with dual-monochromators, dual-mode cuvette ports and top-reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The instrument does endpoint, kinetic, spectrum and area-well scanning read types and is used for DNA/RNA/protein quantification, Bradford, PicoGreen and NanoOrange, ELISA and enzyme kinetics assays.
Thermo Nanodrop One
This instrument is essential for accurately measuring DNA, RNA and protein for direct applications such as real-time qRT-PCR, western blots, high-throughput sequencing, etc. Another benefit is its small footprint, which can easily fit on the benchtop among other equipment. It is also easily cleanable and care is simple, as long as people take a little time.
Analytik Jena UVP Visi-Blue Transilluminator
The amber protective cover blocks blue light transmission and allows visualization of most stains above 500nm. Increased ultraviolet output and intensity. Wide intensity range with variable selection.
High/medium/low intensity settings: The high setting is for UV excitation of fluorophores on gels for routine photography; the medium setting is excellent for viewing and quick single-band excision; and the low setting reduces gel photonicking for positioning and preparing gels, excising multiple bands and focusing for photography.
Improved gel visualization and gel documentation. 460-470nm excitation samples.
Accuris Instruments
This instrument, combined with a special reflector and diffusion filter system, provides exceptional uniformity across the viewing surface. This allows DNA quantification and produces higher-quality images when using the camera system.
Users are protected from the harmful effects of UV radiation by a hinged, UV-blocking safety shield. Suitable for the purpose and quantity of gel to be analyzed. Can read results of electrophoresis gels stained with Ethidium Bromide, SmartGlow LD and PS, Gel Red, Gel Green, SYBR Green, SYBR Safe, Diamond Nucleic Acid Dye
Beckton Dickinson FACSCalibur
The BD Biosciences FACSCalibur instrument is an advanced flow cytometer essential for a wide range of biological applications.
The FACSCalibur boasts a high sample throughput (hundreds per hour), multicolor detection capability, advanced optics and electronics for high sensitivity and resolution, and accurate and reproducible quantification of cellular parameters. These features facilitate precise measurements of cell counts, antigen expression levels, and cell cycle distribution and allow for detecting rare cell populations and subtle changes in cellular characteristics.
Sorvall RC5C Plus
The Sorvall RC-5C Plus Superspeed Centrifuge separates substances by density while controlling temperature. It features an RCF calculator, a dual-purpose integrator, and uses SUVA® refrigerant. Automatic temperature compensation and controlled start/stop processes enhance safety, while run conditions are easily selected and displayed on the front panel. The built-in microcomputer also performs self-tests to prevent malfunctions.
Sorvall Discovery 90 SE Centrifuge
The SORVALL Discovery 90 SE centrifuge is a high-performance macro centrifuge ideal for high-throughput applications. It features a robust 90 kN motor that reaches speeds up to 27,000 rpm and supports rotors with a 90 mL capacity. Key features include customizable acceleration / deceleration settings, automatic rotor verification, imbalance detection, and a secure auto-locking lid. The centrifuge also includes a digital timer, storage tray, and advanced overload protection for enhanced safety and convenience.
Thermo MaxQ 6000
The Thermo Scientific MaxQ 6000 is a stackable incubated shaker for smooth orbital shaking and incubation. It features a triple-eccentric drive and a PID temperature controller, along with alarms for temperature and speed deviations. It's designed for continuous 24-hour operation and is stackable, allowing for efficient use of lab space.