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Middle-Aged Woman with Dark Urine

This case study is based on this question from the ASCLS consumer web page:

I was just wondering if it was possible for you to explain what the following means in a urine analysis:
1. Blood = Large (normal/abnormal? possible reasons if abnormal?)
2. pH = 8 (normal/abnormal? possible reasons if abnormal?)
3. Protein = 100++ (normal/abnormal? possible reasons if abnormal?)
4. Urobilinogen = 12mg (normal/abnormal? possible reasons if abnormal?)
5. Nitrite = Negative (normal/abnormal? possible reasons if abnormal?)
6. Leucocytes (normal/abnormal? possible reasons if abnormal?)


A 35 year old woman went to the doctor complaining of fatigue, shortness of breath, and dark urine. Her skin was very pale, and the whites of her eyes were slightly yellow. She reported recently having a sinus infection which was treated with penicillin. Urinalysis results are:

Color
dark red/brown
Protein
100mg/dL (2+)
Blood
large
Appearance
clear
Glucose
negative
Urobilinogen
12.0 EU
Sp. Gravity
1.020
Ketones
negative
Nitrite
negative
pH
8.0
Bilirubin
negative
Leukocyte
negative

Microscopic
amorphous 2+
hemosiderin granules present

Upon receiving the urinalysis results, the physician orders a blood test for haptoglobin which comes back less than 5 mg/dL (reference range = 26-185 mg/dL).

Questions

1. From what condition is the patient most likely suffering?
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2. If the patient shows signs of jaundice, and there is urobilinogen present, then why is the bilirubin negative?
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3. Would you expect to see the following formed element? Why or why not?

granular cast
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4. Is it possible to have amorphous from the two major crystal types below? Why or why not? What is in this photomicrograph which indicates it definitely could not be from this patient?

ammonium biurate, triple phosphate, and bacteria
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5. Is it possible the protein is a false positive or at least higher than it should be? Should a confirmatory test for protein be performed?
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6. Foods such as red beets cause urine to be dark red. How can the laboratory professional rule this out as a source of false positive on the stix?
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Colleen Carey | Honors Urinalysis | Fall 2005