Welcome to the University of Delaware Sequencing & Genotyping Center
The DNA Sequencing & Genotyping Center provides state of the art genetic analysis for University of Delaware research groups and outside users on a fee-for-service basis. The Applied Biosystems 3130XL Genetic Analyzer housed in the center is a state-of-the-art capillary-based instrument capable of moderate- to high-throughput DNA sequencing and fragment analysis (up to 656 samples/day).
From a pure preparation of DNA (plasmid, PCR product, etc.) the center performs all steps to obtaining DNA sequence (cycle sequencing, purification, capillary electrophoresis, and base calling) and provides sequence data to the investigator from a web-accessible file server. While most submissions are for DNA sequencing, the center runs and provides to the investigator analyzed fragment analysis data. Through our association with the Biomolecular Core Laboratory at A.I. DuPont Hospital for Children we are able to provide Real-time PCR and pyrosequencing services. By request we will perform DNA extraction, DNA purification, and DNA amplification. Contact us for more information concerning any of these services.
The UD DNA Sequencing & Genotyping Center is equipped with the following instrumentation:
• ABI Prism 3130XL Genetic Analyzer – The 3130XL system is a fully automated, fluorescence-based capillary electrophoresis platform that simultaneously analyzes 16 samples. The system provides automated polymer loading, sample injection, separation and detection, and data analysis. The instrument can run unattended for up to 24 hours.
• Apple G5 Server – The Xserve G5 offers dense power in a 1U server with dual 64-bit G5 processors at speeds of 2.3GHz. A UNIX-based server operating system with unlimited clients, up to 1.2TB storage, PCI-X expansion, dual Gigabit Ethernet, fast I/O ports and an optical drive.
• NanoDrop Technologies ND-1000 UV-Vis Spectrophotometer – The NanoDrop® ND-1000 UV-Vis Spectrophotometer enables highly accurate analyses of extremely small samples with high reproducibility. The sample retention system eliminates the need for cuvettes and capillaries. Benefits include full spectrum absorbance (220-750nm) and small sample size (1-2ul).
• ABI GeneAmp PCR System 9700 Thermocycler – High-performance thermocyclers offer fidelity, accuracy, NIST traceable calibration, and fast response to temperature changes. Heating and cooling is thermoelectric, a heated lid provides for oil-free operation.
• Eppendorf Centrifuge 5804R – The 5804R centrifuge is capable of high-speed centrifugation (up to 20,800 x g) of tubes and plates using interchangeable swing-bucket and high-speed fixed-angle rotors. Internal temperature settings range from –9°C to 40°C
Our ABI 3130XL Genetic Analyzer runs a wide variety of sequencing and fragment analysis applications including:
de novo sequencing
Resequencing (mutational profiling)
Epigenetic Analysis via Cytosine methylation
Multi Locus Sequence Typing
Microsatellite analysis
Amplified Fragment-Length Polymorphism (AFLP)
ARISA (Automated Ribosomal Intergenic Spacer Analysis)
SNP screening and validation
Loss of Heterozygosity (LOH)
