The Center for Translational Cancer Research Core Facility has a state-of-the-art Bio-Rad SELDI-TOF-MS
ProteinChip® System. The System is comprised of a ProteinChip Reader (series
PBS II) integrated with ProteinChip Software and a personal computer to analyze
proteins captured on ProteinChip Arrays. The ProteinChip System detects and
accurately calculates the mass of compounds ranging from small molecules and
peptides of less than 1000 Da, up to proteins of 500 kDa or more based on
measured time-of-flight.
You can register or schedule an appointment to use the Bio-Rad ProteinChip System online.
PROTEINCHIP ARRAYS
Bio-Rad's ProteinChip Arrays distinguish this technology from other mass spectrometry-based analytical systems. ProteinChip Arrays provide a variety of surface chemistries that allow researchers to optimize protein capture and analysis. The surface chemistries of the arrays include a series of classic chromatographic chemistries and specialized affinity capture surfaces. Classic chromatographic surfaces include normal phase for generic protein binding; hydrophobic surfaces for reversed-phase capture; cation- and anionexchange surfaces; and immobilized metal affinity capture (IMAC) for metal-binding proteins. Specific proteins of interest can be covalently immobilized on pre-activated surface arrays, enabling customized experiments to investigate antibody-antigen, DNAprotein, receptor-ligand, and other molecular interactions.
Courtesy of Bio-Rad Biosystems, Inc.
THE PROTEINCHIP READER (SERIES PBS II)
The ProteinChip Reader is a laser desorption/ionization time-of-flight mass spectrometer that uses state-of-the-art ion optic and laser optic technology. The laser optics maximize ion extraction efficiency over the greatest possible sample area, and thus increase analytical sensitivity and reproducibility. The Reader's ion optics incorporate a four-stage, time-lag-focusing ion lens assembly that provides precise, accurate molecular weight determination with excellent mass sensitivity.
THE PROTEINCHIP SOFTWARE
Bio-Rad's ProteinChip Software controls all aspects of the ProteinChip Reader and facilitates data collection and analysis. The software uses a Microsoft Windows NT interface, and contains numerous features, including automatic reading of ProteinChip Arrays; multiple spectrum comparison for differential protein display and biomarker discovery; several alternative viewing formats for data; and a user-friendly interface.
LIST OF SERVICES
Fees for services are solely for UD investigators and are subject to change
Service |
Prices |
|---|---|
| Mass Spec use - supervised | $63 per hour |
| Mass Spec use - unsupervised | $42 per hour |
| 1 year unlimited usage | $4,000.00 |
| Protein Chips | $80 each |
| Scientific Consultation | $25 per hour |
ProtienChip is a registered trademark of Bio-Rad Biosystems, Inc.
