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Cumulative Examination in

Analytical Chemistry

September, 2014

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Chemistry and Biochemistry S.D. Brown Home Page

Here you can find the announcement of the cumulative examination in analytical chemistry to be given at 9AM on 13 September 2014.

The topic of the cumulative examination in Analytical Chemistry for September, 2014 is: "Instrumental Characterization of Bacterial Species and Strain." Prof. Brown is the primary examiner for the exam.

A recent Analytical Chemistry article was highlighted in C&E News as a novel way to characterize bacteria. (Anal. Chem. 2014, DOI: 10.1021/ac501075f). Like many good ideas, this one seems to need re-invention (and re-selling) every few years. The basic idea (based on the so-called FAME method, for Fatty Acid Methyl Ester analysis) has been around for at least 35 years, having first been developed for GC of bacteria at Delaware by Myron Sasser in the mid 1970’s, taken to py-MS by Henk Meuzelaar in the late1970’s, then it was modified for routine use by Roger Freeman and Penny Sisson of the Newcastle (UK) Regional Public Health Laboratory in the 1990’s. Many other labs have published some on the topic, but the ones listed above have reduced the methodology to routine practice.

This exam covers instrumental methods for characterization of bacterial species and strain. You should read this paper and research the topic online to see what has been done, what the biomarkers have been, what instrumentation is needed, what data analysis methods are used, and what the limitations are. Finally, from your review, you should form some critical opinions of the recently published work cited above.

Prof. Booksh is the secondary examiner.

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Last updated 18 June 2014