describe atomic absorption & emission spectroscopy (AAS & AES) measurements in stimulus/response framework
describe processes that occur during AAS and AES measurements
list the steps in a typical analysis using AAS or AES measurement
predict the form/presentation of the data collected in a typical AAS or AES measurement
draw the layout of components in the prototype (discussed in class) AAS and AES spectrometers
draw and explain the operation of prototype AAS and AES spectrometer components
calculate the properties (e.g., bandwidth, intensity) of the analyte signal passed by the component
draw the layout of AAS and AES spectrometers using alternative components, layouts
discuss the relative advantages and disadvantages of components that have similar function
use LLSR to find calibration line relating AAS or AES signal to concentration, e.g., Hg in tuna,
compute the standard deviation & confidence interval of concentrations found using LLSR calibration lines
use a single standard addition to find the analyte concentration in a complex sample matrix
use standard addition with LLSR to find the analyte concentration in a complex sample matrix
compute the standard deviation & confidence interval of concentrations found using standard addition
describe the types of noise that can be observed in the signal collected by AAS and AES spectrometers
describe the interference types, their sources and remedies for AAS and AES measurements
discuss the relative advantages and disadvantages of AAS and AES measurements
describe electronic molecular absorption & fluorescence spectroscopy (EMAS & MFS) measurements in stimulus/response framework
describe and illustrate processes that occur during EMAS & MFS measurements
relate electronic transitions to the structure of the chromophore
list the steps in a typical analysis using EMAS & MFS measurement
predict the form/presentation of the data collected in a typical EMAS & MFS measurement
draw the layout of components in the prototype (discussed in class) EMAS & MFS spectrometers
draw and explain the operation of prototype EMAS & MFS spectrometer components
calculate the properties (e.g., bandwidth, intensity) of the analyte signal passed by the component
draw the layout of EMAS & MFS spectrometers using alternative components, layouts
discuss the relative advantages and disadvantages of components that have similar function
derive Beer’s law
calculate the fluorescence quantum yield and lifetime
use LLSR to find calibration line relating EMAS & MFS signals to concentration, e.g., PAH in water
compute the standard deviation & confidence interval of concentrations found using LLSR calibration lines
use a single standard addition to find the analyte concentration in a complex sample matrix
use standard addition with LLSR to find the analyte concentration in a complex sample matrix
compute the standard deviation & confidence interval of concentrations found using standard addition
calculate the standard deviation of the analyte concentration from the standard deviation of the EMAS or MFS measurements (transmittance or intensity)
describe the types of noise that can be observed in the signal collected by EMAS & MFS spectrometers
discuss the relative advantages and disadvantages of EMAS & MFS measurements
describe vibrational molecular absorption & scattering spectroscopy (IRAS & RSS) measurements in stimulus/response framework
describe and illustrate processes that occur during IRAS & RSS measurements
relate vibrational transitions to the structure of the chromophore
compute number, frequencies and energies of vibrational transitions expected
predict if a vibration will be IRAS or RSS active
list the steps in a typical analysis using IRAS & RSS measurement
predict the form/presentation of the data collected in a typical IRAS & RSS measurement
draw the layout of components in the prototype (discussed in class) IRAS & RSS spectrometers
draw and explain the operation of prototype IRAS & RSS spectrometer components
calculate the properties (e.g., bandwidth, intensity) of the analyte signal passed by the component
draw the layout of IRAS & RSS spectrometers using alternative components, layouts
discuss the relative advantages and disadvantages of components that have similar function
use LLSR to find calibration line relating EMAS & MFS signals to concentration, e.g., CNT in solvent
compute the standard deviation & confidence interval of concentrations found using LLSR calibration lines
use a single standard addition to find the analyte concentration in a complex sample matrix
use standard addition with LLSR to find the analyte concentration in a complex sample matrix
compute the standard deviation & confidence interval of concentrations found using standard addition
calculate the standard deviation of the analyte concentration from the standard deviation of the IRAS & RSS measurements (transmittance or intensity)
describe the types of noise that can be observed in the signal collected by IRAS & RSS spectrometers
discuss the relative advantages and disadvantages of molecular optical spectroscopy measurements