Department of Chemistry & Biochemistry
Room 312 Drake Hall
University of Delaware
Newark, DE  19716
Telephone: 302-831-0786
Fax: 302-831-6335
e-mail:  bahnson@udel.edu
URL:  http://www.udel.edu/chem/bahnson

Professional Experience:
September 2004 - Present
University of Delaware, Department of Chemistry & Biochemistry,
Associate Professor 

September 1998 - September 2004
University of Delaware, Department of Chemistry & Biochemistry,
Assistant Professor 

July 1994 - July 1998
Brandeis University, Structural Biology Laboratory, Postdoctoral Fellow 
Sponsors: Gregory A. Petsko and Dagmar Ringe
Area of study: x-ray crystallography

January 1994 - June 1994
Williams College, Department of Chemistry,
Visiting Assistant Professor
Teaching experience: a senior honors seminar course in
biochemistry & molecular biology and general chemistry labs 

September 1990 - December 1993
University of California, Berkeley, Department of Chemistry

National Institute of General Medical Sciences, Postdoctoral Fellow, 
Sponsor: Judith P. Klinman.
Area of study: enzyme kinetics and molecular biology 

Education:
September 1986 - August 1990
Brown University, Department of Chemistry, Ph.D.
Advisor: Vernon E. Anderson.
Thesis title: "The Chemical Mechanism of Crotonase"
Teaching experience: teaching assistant for general
and organic chemistry 

September, 1982 - May, 1986
University of Massachusetts, Amherst
B.S. Chemistry, B.S. Biochemistry, Cum Laude

Invited Talks:

1. Symposium lecture, ACS Biological Division, San Francisco, CA, August 1997
2. Dupont Stine-Haskell Research Center, Newark, DE, March 1999
3. Dupont Experimental Station, Wilmington, DE, September 2000
4. Symposium lecture, Biophysical Society Meeting, Boston, MA, February 2001
5. Villanova University, Dept. of Chemistry, March 2001
6. Wake Forest Univ. School of Medicine, Dept. of Biochemistry, March 2001
7. Delaware Valley Enzymology Club, Wilmington, DE, May 2001
8. Case Western University, Department of Biochemistry, Cleveland, OH, July 2001
9. Rutgers University, Department of Chemistry, Newark, NJ, April 2002
10. Lexicon Pharmaceutical, Princeton, NJ, December 2002
11. Delaware Biotechnology Institute, Newark, DE, December 2002
12. North Carolina State University, Dept. of Biochemistry, Raleigh, NC, February 2003
13. University of Toledo, Dept. of Chemistry, Toledo, Ohio, March 2003
14. University of Iowa, Dept. of Chemistry, Iowa City, Iowa, April 2003
15. University of Maryland, Dept. of Chem. and Biochem., College Park, MD, April 2003
16. Symposium lecture, Pittsburgh Diffraction Conf., Rutgers Univ., New Brunswick, NJ, Nov. 2003
17. First International Conference on Paraoxonases, Univ. of Michigan, April 22-24 2004.
18. FASEB Conference on Phopholipases, Pine Mountain Georgia, July 17-22, 2004.
19. 2nd Int. Conf. on PLA2 & 8th Int Congress PAF and Lipid Mediators, Berlin, Germany, Oct 6-9, 2004.
20. Colgate University, Dept. of Chemistry, Hamilton New York, Nov. 2, 2004.
21. University of Pennsylvania, Institute for Environmental Med., Philadelphia, PA, April 8, 2005.

Recruiting Visits and Seminars:

1. Washington College, Dept. of Chemistry, Chestertown, MD, October 2001
2. Shippensburg University, Dept. of Chemistry, Shippensburg, PA, November 2001
3. College of Notre Dame, Dept. of Chemistry, Baltimore, MD, February 2002
4. Saint Francis College, Dept. of Chemisty, Brooklyn, NY, April 11, 2005.

Poster Presentations:

1. Enzymes, Coenzymes and Metabolic Pathways Gordon Conference, July 1998
2. 17th Enzyme Mechanisms Conference, Marco Island, FL, January 2001
3. Biophysical Society Meeting, Boston, MA, February 2001
4. 4th European Symposium of Protein Society, Paris, France, April 2001
5. Frontiers of Struct. Biol., Keystone Symposium, Breckenridge, CO, January 2002
6. USAMRICD Bioscience Review, Hunt Valley, MD, June 2002
7. FASEB Summer Research Conf., Phospholipases, Tucson, AZ, June 2002
8. Enzymes, Coenzymes and Metabolic Pathways Gordon Conference, July 2002
9. 18th Enzyme Mechanisms Conference, Galveston, TX, January 2003

Publications:
B. J. Bahnson and V. E. Anderson* (1989) Isotope effects on the crotonase reaction, Biochemistry 28, 4173-4181.

V. E. Anderson*, B. J. Bahnson, I. D. Wlassics, and C. T. Walsh (1990) The reaction of acetyldithio-CoA, a readily enolized analog of acetyl-CoA with thiolase from Zoogloea ramigera, Journal of Biological Chemistry 265, 6255-6261.

B. J. Bahnson and V. E. Anderson* (1991) Crotonase-catalyzed beta-elimination is concerted: A double isotope effect study, Biochemistry 30, 5894-5906.

B. J. Bahnson, D.-H. Park, K. Kim, B. V. Plapp, and J. P. Klinman* (1993) Unmasking of hydrogen tunneling in the horse liver alcohol dehydrogenase reaction by site-directed mutagenesis, Biochemistry 32, 5503-5507.

R. L. D'Ordine Jr., B. J. Bahnson, P. Tonge, and V. E. Anderson* (1994) Enoyl coenzyme-A hydratase catalyzed exchange of the alpha-protons of coenzyme-A thiol esters: A model for an enolized intermediate in the enzyme catalyzed elimination?, Biochemistry 33, 14733-14742.

B. J. Bahnson and J. P. Klinman* (1995) Hydrogen tunneling in enzyme catalysis, Methods in Enzymology (D. L. Purich ed.), 249, 373-397.

B. J. Bahnson, T. D. Colby, J. K. Chin, B. M. Goldstein, and J. P. Klinman* (1997) A link between protein structure and enzyme catalyzed hydrogen tunneling, PNAS 94, 12797-12802.

T. D. Colby, B. J. Bahnson, J. K. Chin, J. P. Klinman, and B. M. Goldstein* (1998) Active site modifications in a double mutant of liver alcohol dehydrogenase: structural studies of two enzyme-ligand complexes, Biochemistry 37, 9295-9304.

C. J. Jeffery, B. J. Bahnson, W. Chien, D. Ringe and G. A. Petsko* (2000) The crystal structure of rabbit phosphoglucose isomerase; a glycolytic enzyme that moonlights as neuroleukin, autocrine motility factor and differentiation mediator, Biochemistry, 39, 955-964.

Y. H. Pan,  T. M. Epstein, M. K. Jain, and B. J. Bahnson* (2001) Five coplanar anion binding sites on one face of phospholipase A2:  relationship to interface binding, Biochemistry, 40, 609-617.

T. M. Epstein, Y. H. Pan, S. P. Tutton, M. K. Jain, and B. J. Bahnson* (2001) The basis for k*cat impairment in prophospholipase A(2) from the anion-assisted dimer structure, Biochemistry, 40, 11411-22

B. J. Bahnson*, V. E. Anderson, and G. A. Petsko (2002) Structural Mechanism of Enoyl-CoA Hydratase: Three Atoms from a Single Water are Added in Either an E1cb Stepwise or Concerted Fashion, Biochemistry 41, 2621-2629.

R. L. D'Ordine, J. Pawlak, B. J. Bahnson V. E. Anderson* (2002) Polarization of Cinnamoyl-CoA Substrates Bound to Enoyl-CoA Hydratase: Correlation of 13C NMR with Quantum Mechanical Calculations and Calculation of Electronic Strain Energy, Biochemistry 41, 2630-2640. 

J. Pawlak, B. J. Bahnson and V. E. Anderson* (2002) DFT/PM3 Study of the enoyl-CoA hydratase catalyzed reaction, Nukleonika, 47 (Supplement 1): 115-155.

Y. H. Pan, B. Z. Yu, A. G. Singer, F. Ghomashchi, G. Lambeau, M. H. Gelb, M. K. Jain, and B. J. Bahnson* (2002) Crystal Structure of Human Group-X Secreted Phospholipase A2; Electrostatically Neutral Interfacial Binding Surface Targets Zwitterionic Membranes,Journal of Biological Chemistry, 277, 29086-29093.

C. Ceccarelli, N. Grodsky, N. Aryaratne, R.F. Colman and B.J. Bahnson* (2002) Crystal Structure of Porcine Mitochondrial NADP+-dependent Isocitrate Dehydrogenase Complexed with Mn2+ and Isocitrate Insights into the Enzyme Mechanism, Journal of Biological Chemistry, 277, 43454-43462.

Y. Pan, B. Z. Yu, O. G. Berg, M. K. Jain and B. J. Bahnson* (2002) Crystal Structure of Phospholipase A2 in Complex with the Hydrolysis Products of Platelet Activating Factor: Equilibrium Binding of Fatty Acid and Lysophospholipid-Ether at the Active Site May Be Mutually Exclusive, Biochemistry, 41, 14790-14800.

P. M. Tessier, H. R. Johnson, R. Pazhianur, B. W. Berger, J. L. Prentice, B. J. Bahnson, S. I. Sandler, and A. M. Lenhoff* (2002) Predictive Crystallization of Ribonuclease A via Rapid Screening of Osmotic Second Virial Coefficients, Proteins: Structure, Function and Genetics, 50, 303-311. 

B. P. Smart, Y. H. Pan, A. K. Weeks, J. G. Bollinger, B. J. Bahnson* and M. H. Gelb* (2004) Inhibition of the Complete Set of Mammalian Secreted Phospholipases A2 by Indole Analogs: A Structure-Guided Study Bioorganic and Medicinal Chemistry, 12, 1737-1749.

C. Ceccarelli, Z. X. Liang, M. Strickler, G. Prehna, B. M. Goldstein, J. P. Klinman, and B. J. Bahnson* (2004) Crystal Structure and Amide H/D Exchange of Binary Complexes of Alcohol Dehydrogenase from B. stearothermophilus: Insights to Thermostability and Cofactor Binding Biochemistry, 43, 5266-5277.

D. T. Yeung, D. Josse, J. D. Nicholson, A. Khanal, C. W. McAndrew, B. J. Bahnson, D. E. Lenz, and D. M. Cerasoli* (2004) Structure/Function Analyses of Human Serum Paraoxonase (HuPON1) Mutants Chosen from a DFPase-like Homology Model Biochim. Biochem. Acta, 1702, 67-77. 

B. J. Bahnson* (2004) An atomic-resolution mechanism of 3-hydroxy-3-methylglutaryl-CoA synthase
Proc Natl Acad Sci USA 101, 16399-400.

B. J. Bahnson* (2005) Structure, Function and Interfacial Allosterism in Phospholipase A2: Insight from the Anion-Assisted Dimer Archives of Biochem. and Biophys., 433, 96-106.

B. Z. Yu, Y. H. Pan, M. Janssen, B. J. Bahnson*, and M. K. Jain* (2005) Kinetic and Structural Properties of Disulfide Engineered Phospholipase A2: Insight into the Role of Disulfide Bonding Patterns Biochemistry, 44, 3369-79.

C. J. Blamey, C. Ceccarelli, U. P. Naik, and B. J. Bahnson* (2005) The Crystal Structure of Calcium- and Integrin-Binding Protein 1: Insights into Redox Regulated Functions, Protein Science, 14, 1214-1221.

B. Berger, C. J. Blamey, U. P. Naik, B. J. Bahnson, and A. M. Lenhoff* (2005) Roles of Additives and Precipitants in Crystallization of Calcium- and Integrin-Binding Protein Crystal Growth & 
Design, 5, 1499-1507.