BISC411

               EXPERIMENTAL MOLECULAR BIOLOGY OF THE CELL

Welcome to our course homepage. From this docking point you will be able to access the laboratory schedule,  protocols, etc. for our course. There will not be a laboratory manual. Please take some time to
familiarize yourself with the background information about the course, the course philosophy, etc. We will be
having extensive discussions related to the actual lab protocols as part of our course so you should not be
concerned if your initial reading of the protocols seems unclear. I hope you will enjoy this semester and find the course a worthwhile experience.

Professor Information

Course Policies

Course Objectives and Background

General Schedule and Grading Criteria

Guidelines for the Laboratory Reports

Using the PDB to Examine T4 Lysozyme Structure

Background on Restriction Mapping and Pencil Exercise/Questions

Mini-Prep Protocol and Measurement of DNA yield

Competent Cells and Bacterial Transformation Protocols

Information About Alkaline Lysis For Plasmid DNA Purification

Restriction Enzyme Digestion and Agarose Gel Protocols

Determination of Lysozyme Activity Standard

Batch Induction Protocol

Ion-Exchange Purification Protocol
 
Background Information About Polyacrylamide Gel Electrophoresis

Polyacrylamide Gel Electrophoresis Analysis Protocol

Questions to Consider about the Journal Articles, Sequences, and other Information Related to the Plasmids and Proteins We Are Using

Measuring Total Protein Concentration (Biuret Reaction) and Units of Lysozyme Activity Protocols