ULTRAMICROTOMY AND STAINING OF THIN SECTIONS

---

 

---

 

Lecture Notes for Ultramicrotomy and Staining

Ultramicrotomy	Fine Adjustments	Chatter and Scratches
Diamond Knives	Interference Colors and Section Thickness	Tissue Folds and Boat Water Contamination
Making Glass Knives	Thick Section, Thin Section	Positive Staining of Thin Sections
Evaluation of Knife Edge	Troubleshooting 1	Staining Grids with Uranyl Acetate
Boats and Troughs	Troubleshooting 2	Uranyl Acetate Contamination
Trimming the Specimen Block	Troubleshooting 3	Staining Grids with Lead Citrate
Location of Tissue in Thick Sections	Retrieval of Sections on Grids	Lead Contamination
Reichert Ultracut E Ultramicrotome	Chatter	Student Micrograph
Alignment of Block Face with Knife Edge	Scratches and Holes	Grid Types & Mesh Sizes