Metabolism Review
ATP is produced via:
- substrate-level phosphorylation when high energy phosphate intermediates
are formed and are transferred to ADP to produce ATP. Examples of
this are found in glycolysis and the TCA cycle.
- electron-transport phosphorylation when electrons move down the electron
transport chain and chemiosmosis occurs. The electrons were picked
up by electron carriers (NAD in glycolysis and the TCA cycle; FAD in the
TCA cycle) and brought to the electron transport chain.
- photophosphorylation as a result of photosynthesis (which also involves
an electron tansport chain). Photosynthesis has light and dark reactions.
In the light reactions ATP (via photophosphorylation) and reduced NADP
are formed. These are used in the dark reactions (the Calvin Cycle)
to reduce CO2 to the level of carbohydrate.
If we distinguish organisms based on their energy source:
- Heterotrophs obtain energy from the oxidation of organic compounds
(meaning that organic compounds function as e- donors). The
final e- acceptor determines whether these organisms are carrying out
- respiration (if oxygen is the terminal e- acceptor)
- anaerobic respiration (if an inorganic compound other than oxygen is
the terminal e- acceptor
- fermentation (if an organic compound is the final e- acceptor)
- Lithotrophs obtain energy from the oxidation of inorganic compounds
(meaning that inorganic compounds function as e- donors). Two examples
are
- nitrifying bacteria which use NH3 or NO2- as energy sources (e-
donors)
- sulfur-oxidizing bgacteria which can use H2S or S as energy sources
(e- donors)
Remember -These organisms use oxygen as the terminal e- acceptor and
are therefore carrying out respiration
- Phototrophs obtain energy from light (light provides the energy to
eject an e- from a chlorophyll molecule)
- oxygenic photosynthesis is carried out by green plants, algae and cyanobacteria.
These organisms split water into hydrogen (which they use for reducing
power) and oxygen (which is released as O2). As a result of photosynthesis
(light reactions) by these organisms ATP and reduced NADP are formed.
The ATP and reduced NADP are used to reduce CO2 in what are called the
dark reactions of photosynthesis (= Calvin Cycle). The reduced NADP provies
the H's necessary to convert CO2 ---> CH2O (carbohydrate) and the ATP
provides the energy.
- anoxygen photosynthesis is carried out by purple and green bacteria.
These organisms do not split water but can, for example, use H2S as an
e- donor (reducing power). Oxygen is not produced in this process.
Let's put terms together using heterotrophs as an example:
- A heterotrophic organism that is carrying out respiration
- these organisms are using organic compounds as e- donors and oxygen
as a terminal electron acceptor
- The organisms are carrying out
- glycolysis
- the TCA cycle
- the electron transport system
- They are obtaining ATP via
- SLP (glycolysis and the TCA cycle)
- oxidative phosphorylation (in e- transport system)
- A heterotrophic organism that is carrying out anaerobic respiration
- these organisms are using organic compounds as e- donors and inorganic
compounds other than oxygen as a terminal electron acceptor. Note that
if NO3 sserves as the terminal e- acceptor the organism is said to be carrying
out denitrification and if SO4 serves as the terminal e- acceptor the organism
is said to be carrying out sulfate reduction.
- The organisms are carrying out
- glycolysis
- the TCA cycle
- the electron transport system
- They are obtaining ATP via
- SLP (glycolysis and the TCA cycle)
- oxidative phosphorylation (in e- transport system)
- A heterotrophic organism that is carrying out fermentation
- these organisms are using organic compounds as e- donors and organic
compounds as terminal e- acceptors
- The organisms are carrying out
- They are obtaining ATP via
SLP only (glycolysis)
- Review: how many molecules of ATP are produced when an organism
is carrying out aerobic respiration compared as opposed to respiration:
# of
ATPs/molecule of glucose obtained using aerobic respiration vs. fermentation