B300 - Exam 2 - Sample Questions

B300 Exam 2 - Sample Questions

1. What happens in the Calvin Cycle?

a. ATP and reduced NADP are produced

b. glucose is oxidized

c. ATP is produced

d. CO2 is fixed

2. Given the following Redox Couples and their E'o. Indicate the correct order in which movement of electrons will occur.

Pair A = +0.44; Pair B = -0.42; Pair C = 0+.815

a. A -> B -> C

b. A -> C -> B

c. B -> C -> A

d. B -> A -> C

3. In chemiosmosis energy for moving protons outside the membrane is provided by:

a. protons moving down the electron transport system

b. electrons moving down the electron transport system

c. the breakdown of ATP ---> ADP

d. the production of ATP

4. Consider fermentation and aerobic respiration. In both of these processes:

a. reduced NAD and reduced FAD are produced

b. the energy source is completely oxidized

c. the energy source is partially oxidized

d. substrate level phosphorylation occurs

5. An organism that uses NO2- as an electron donor and O2 as an electron acceptor is an example of a:

a. chemolithotroph

b. chemoheterotroph

c. photoautotroph

d. photoheterotroph

6. Assume you isolate an organism that is capable of reducing carbon dioxide (CO2) to methane (CH4). What process is this organism carrying out?

a. aerobic respiration

b. anaerobic respiration

c. fermentation

d. anaerobic respiration and fermentation

7. Consider aerobic respiration. Which pathways and cycles occur in this process?

a. glycolysis

b. glycolysis and the Krebs cycle

c. glycolysis, the Krebs cycle and the electron transport chain

d. the Krebs cycle and the electron transport chain

8. In transcription:

a. DNA is used as a template for the synthesis of DNA

b. DNA is used as a template for the synthesis of RNA

c. RNA is used as a template for the synthesis of DNA

d. RNA is used as a template for the synthesis of RNA

9. A competent cell is necessary for ____________ to occur.

a. transformation

b. conjugation

c. generalized transduction

d. specialized transduction

10. Which of the following contains intervening sequences?

a. eucaryotic hnRNA

b. eucaryotic mRNA

c. procaryotic DNA

d. procaryotic mRNA

11. The replica plating technique is used to:

a. detect auxotrophic mutants

b. detect autotrophic mutants

c. determine if a chemical agent is a carcinogen

d. directly detect antibiotic resistant mutants

12. The Hershey-Chase experiment:

a. proved that DNA was the transforming principle

b. proved that DNA synthesis was in the 5' -> 3' direction

c. proved that DNA was the genetic material

d. proved that a bacteriophage can infect bacterial cells

13. The F factor is made of:

a. protein

b. lipid

c. carbohydrate

d. nucleic acid

14. Host enzymes that protect the host from infection by degradation of DNA from infecting bacteriophages are called:

a. protection endonucleases

b. protection exonucleases

c. restriction endonucleases

d. restriction exonucleases

15. What is an open reading frame?

a. a reading frame that is interrupted by a stop codon

b. a sequence of DNA that has less than 10 codons

c. a sequence of DNA that contains genes that code for a protein

d. a sequence of RNA that contains genes that code for a protein

16. The simplest transposable element is called a(n):

a. insertion sequence

b. composite transposon

c. plasmid

d. episome

17. Assume an F+ x F- mating. What is the recipient cell at the end of the mating?

a. F+

b. F-

c. F' (F prime)

d. Hfr

18. What is the major difference between an F+ cell and an HFr cell?

a. The F+ cell has the F factor present, the HFr cell does not.

b. The HFr cell is a genetic recombinant, the F+ cell is not.

c. The HFr cell has the F factor present, the F+ cell does not.

d. The location of the F factor

19. Which of the following techniques is used to synthesize large quantities of a DNA fragment without cloning it? a. electrophoresis

b. PCR

c. hydrolysis

d. fermentation

20. In DNA replication, __________ are responsible for unwinding the DNA.

a. DNA dependent DNA polymerases

b. helicases

c. primases

d. restriction endonucleases

Also... Don't forget to go over the genetics problems that we reviewed in class.