Techniques in Biological Electron Microscopy 

---

---

  Techniques in Biological Electron Microscopy (B 617) is a graduate -level laboratory course offered each spring semester to 10 students by permission of intructor only. Students obtain hands-on experience in preparation of tissue samples for Transmission and Scanning Electron Microscopy, recording of images by microscopy and presentation of results in a publication format.   For More Information Contact-   Dr. Roger C. Wagner (instructor)    28499@udel.edu
This page was prepared by Anuj Parikh, AS98, JMC2002
 
 

---

 
 

Lecture Topics 
(click on topic to review notes)

Preparation of Tissues for Transmission Electron Microscopy	Ultrastructural Cytochemistry	Freeze-Fracture and Etching
Ultramicrotomy and Staining of Thin Sections	Tracers and Labels for Transmission EM	Morphometric Techniques
Transmission Electron Microscopy	Negative Staining and Shadow Casting	Three-Dimensional Reconstruction
Preparation of Tissues for Scanning Electron Microscopy	Corrosion Casting	High Resolution Radioautography
Scanning Electron Microscopy	Stereomicroscopy	Digital Image Processing and Analysis
Photography and Darkroom Techniques	Cryofixation	Analytical Electron Microscopy